Immunogold labeling in scanning electron microscopy
نویسندگان
چکیده
منابع مشابه
Immunogold labelling for scanning electron microscopy.
Scanning electron microscopes are useful biological tools that can be used to image the surface of whole organisms, tissues, cells, cellular components and macromolecules. Processes and structures that exist at surfaces can be imaged in pseudo or real 3D at magnifications of anything from about x10 to x1,000,000. Therefore a whole multicellular organism, such as a fly, or a single protein embed...
متن کاملImmunogold labeling of amelogenin in developing porcine enamel revealed by field emission scanning electron microscopy.
The present study describes a method using immunohistochemical labeling in combination with high-resolution imaging (field emission scanning electron microscopy) to investigate the spatial localization of amelogenins on apatite crystallites in developing porcine enamel. Cross-sections of developing enamel tissue from freeze-fractured pig third molar were treated with antiserum against recombina...
متن کاملImmunogold Labeling of Cultured Cells and Virus Particles for Electron Microscopy and Cryo-Electron Microscopy Applications
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) were first realized in the early 1980s [1] and mid-1990s [2, 3]. Since then both methods have quickly become a powerful tools in structural biology. The major advantage is that they allow for the acquisition of images of biological specimens persevered in their native state after they are plunge-frozen onto carbon-coated ...
متن کاملImmunogold scanning electron microscopy can reveal the polysaccharide architecture of xylem cell walls
Immunofluorescence microscopy (IFM) and immunogold transmission electron microscopy (TEM) are the two main techniques commonly used to detect polysaccharides in plant cell walls. Both are important in localizing cell wall polysaccharides, but both have major limitations, such as low resolution in IFM and restricted sample size for immunogold TEM. In this study, we have developed a robust techni...
متن کاملFat uptake evaluation in fried fish fillet by using Scanning Electron Microscopy (SEM)
The aim of this study was to investigate the effects of breading materials and two different frying oils on the fat up-take in the fish fillets during frying process. Breaded and non-breaded black pomfret (Parastromateus niger) fillets were fried in sunflower oil and palm olein. Fat content by Soxhelet method and scanning electron microscopy (SEM) was studied. Results from Soxhelet method showe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Histochemistry and Cell Biology
سال: 1996
ISSN: 0948-6143,1432-119X
DOI: 10.1007/bf02473246